Page 3/11 since enzymes are complex proteins, any factors that affect protein structure will also affect enzyme activity these factors include ph, temperature, and the presence of other. 48 factors affecting enzyme activity by john eed (biology 1151) abstract: e studied the effect of temperature, enzyme concentration and ph on enzyme activity. This lesson focuses on enzyme activity we'll go over what an enzyme is and how it's used in the body we'll also look at the different factors that affect enzyme activity, both positively and.
1 investigation 2-13 enzyme activity how do abiotic or biotic factors influence the rates of enzymatic reactions background enzymes are the catalysts of biological systems. - investigating enzyme activity aim: to investigate how the concentration of hydrogen peroxide effects the rate of reaction of an enzyme (catalase) variables: these factors could effect the rate of reaction on an enzyme: ph concentration temperature surface area ph - enzymes function at different ph values. This is an experiment to examine how the concentration of the substrate hydrogen peroxide affects the rate of reaction of the enzyme catalase. In this classroom lab, students will explore the characteristics of a particular enzyme and the factors that affect its ability to catalyze a reaction students will look at how the enzyme catalase (source of catalase is chicken liver) affects the break down of hydrogen peroxide into water and oxygen.
Based on these data, how does enzyme activity vary with concentration because the disks were all soaked in the same amount of catalase, the speed of the reaction increased in other words, the number of machines that break down hydrogen peroxide was increased, and the amount of hydrogen peroxide was kept constant. (although not all) factors that can influence enzyme activity 1 effect of enzyme concentration an enzyme molecule is analogous to a worker. Part a - catalase concentration if there is an excess in hydrogen peroxide and the concentration of catalase is increased, the rate of catalysis will also be increased however, if there's a shortage of hydrogen peroxide, the increase of catalase concentration will have no effect in the reaction therefore, the activity of catalase under greater concentrations of it, is dependent on the. Factors affecting enzymes 1 factors affecting enzymecatalyzed reactions 2 factors affecting enzyme-catalyzed reactions 1- effect of substrate concentration (single-substrate reactions) 2- effect of inhibitors 3- effect of ph 4- effect of temperature 5- effect of pressure 6- effect of water. Controlling these factors in a cell is one way that an organism regulates its enzyme activity and so its metabolism changing the concentration of a substance only affects the rate of reaction if it is the limiting factor : that is, it the factor that is stopping a reaction from preceding at a higher rate.
Enzyme action: testing catalase activity biology with vernier 6a - 3 part i testing the effect of enzyme concentration 3 place three test tubes in a rack and label them 1, 2, and 3. The extent at which environmental factors affect the rate of catalase activity was discovered in this lab the assay system, in which a filter paper disc was dipped into the enzyme and submerged using a stirring rod in a test tube filled with 20ml of hydrogen peroxide, was used to test several enzyme factors. Enzyme activity how do abiotic or biotic factors influence the rates of enzymatic reactions c peroxidase and catalase) using peroxidase, students will develop.
The marked difference in catalase activity under circumstances where temperature is the only variable factor may be due, of course, to the fact that its optimum, unlike that of other enzymes. The active site, or which blocks access of the substrate to the active site, will affect enzyme activity such environmental factors include salt concentration, ph, temperature, activators, and inhibitors. In this exercise you will study the enzyme catalase, which speeds up the breakdown of hydrogen peroxide, h 2 o 2 , (a common waste product of cellular metabolism) into water and oxygen catalase. Colder temperatures proved to slow down the reaction of catalase and hydrogen peroxide, this was due to the fact that at colder temperatures enzymatic and substrate activity slows at warmer temperatures the reaction occurred quickly however when extreme heat was tested on the reaction, it occurred very slowly.
This lab shows the affects these factors have on the rate of reaction between catalase, an enzyme found in potatoes, and hydrogen peroxide, the substrate the specific enzyme that was studied during this lab was catalase. Lab : factors influencing enzyme activity background catalase might also take part in some of the many oxidation reactions going on in all cells the primary.
Aim to investigate the effects of ph on enzyme activity research question to what will ph levels 1,7,12 affect the rate at which liver catalase catalyzes the decomposition of 10ml of hydrogen peroxide (h2o2) to water (h2o) and oxygen (o2) exemplified by the volume of foam reached in 1 minute hypothesis since the optimum ph of liver catalase is. 420 peroxidase activity about 75 cc the residual yellow liquid, after clarification in a centrifuge, was treated at 10 with 5 volumes of absolute alcohol. Determine the factors that affect the rate of enzyme activity explain how each factor (ie ph, temperature, concentration of enzyme/substrate) alters the rate of enzyme activity formulate a hypothesis about the environmental factors that will alter the rate of enzyme activity. Enzymes, like catalase, are proteins that speed up chemical reactions by reducing the amount of energy the reaction needs to take placespecifically, an enzyme reduces the activation energy needed to convert a certain substrate into a particular product.